Recombinant:Article Title: AZ14289671 is a highly selective and blood-brain barrier penetrant irreversible TKI that targets EGFRExon20 insertions
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-EGF Receptor (Tyr1068) Antibody Cell Signaling Technology Cat#2234; RRID:AB_331701 EGF Receptor (D38B1) XP Rabbit mAb Cell Signaling Technology Cat#4267; RRID:AB_2246311 Phospho-p44/42 MAPK (Erk1/2) (Thr202/ Tyr204) Antibody Cell Signaling Technology Cat#9101; RRID:AB_331646 p44/42 MAPK (Erk1/2) Antibody Cell Signaling Technology Cat9102; RRID:AB_330744 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat#4060; RRID:AB_2315049 Akt Antibody Cell Signaling Technology Cat#9272; RRID:AB_329827 Phospho-S6 Ribosomal Protein (Ser235/ 236) (D57.2.2E) XP Rabbit mAb Cell Signaling Technology Cat#4858; RRID:AB_916156 S6 Ribosomal Protein (54D2) Mouse mAb Cell Signaling Technology Cat#2317; RRID:AB_2238583 Biological Samples LU3075 Crown Bioscience N/A LU0387 Crown Bioscience N/A Chemicals, peptides, and recombinant proteins AZ14289671 AstraZeneca N/A Mobocertinib AstraZeneca N/A Zipalertinib AstraZeneca N/A Sunvozertinib AstraZeneca N/A Critical commercial assays HTRF Human Phospho-EGFR (Tyr1068) Detection Kit Revvity Part #: 64EG1PEG HTRF Human and Mouse Phospho-ERK (Thr202/Tyr204) Detection Kit Revvity Part #: 64AERPET Deposited data Original code used to compare the growth rate inhibition or potency of the compounds tested This paper https://doi.org/10.5281/zenodo.15534119 Experimental models: Cell lines NCI-H2073 ATCC CRL-5918; RRID:CVCL_1521 LXF2478 Oncotest N/A .. HaCaT Professor Norbert E. Fusenig in Deutsches Krebsforschungszentrum (DKFZ) N/A NCI-H2073 EGFRD770-N771InsSVD AstraZeneca Generated as previously described by Floc’h et al.3 NCI-H2073 EGFRV769-D770InsASV AstraZeneca Generated as previously described by Floc’h et al.3 NCI-H2073 EGFRD770-N771InsNPG AstraZeneca Generated as previously described by Floc’h et al.3 Experimental models: Organisms/strains Mouse: SCID: C.B-17/IcrHanHsd-Prkdcscid Envigo UK N/A Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo UK N/A Mouse: Balb/c Nude: BALB/cNj-Foxn1nu/ Gpt GemPharmatech Co., Ltd N/A (Continued on next page) Cell Reports Medicine 6, 102305, September 16, 2025 e1 Article ll OPEN ACCESS
Article Title: Beta cells intrinsically sense and limit their secretory activity via mTORC1-RhoA signaling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig anti-insulin Primary Agilent Cat# IR002, RRID:AB_2800361 Mouse anti-glucagon Santa Cruz Biotechnology Cat# sc-514592; RRID:AB_2904166 Rabbit anti-phospho-S6 ribosomal protein (Ser240/244) Cell Signaling Technology Cat# 5364; RRID:AB_10694233 Rat anti-puromycin Antibody, clone 17H1 Sigma-Aldrich Cat# MABE341, RRID:AB_3677409 Rabbit phospho-Myosin Light Chain 2 (Ser19) Antibody Cell Signaling Technology Cat# 3671, RRID:AB_330248 Rabbit phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb Cell Signaling Technology Cat# 2855; RRID:AB_560835 Mouse anti-F-actin antibody [NH3] Abcam Cat# ab205; RRID:AB_302794 Phalloidin-iFluor 594 Abcam Cat# ab176757; RRID: AB_3695680 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb Cell Signaling Technology Cat# 4060, RRID:AB_2315049 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb Cell Signaling Technology Cat# 2535, RRID:AB_331250 Purified Mouse Anti-E-Cadherin BD Biosciences Cat# 610181, RRID:AB_397580 Biological samples Cadaveric human islets Prodo labs N/A SC-islets Kadimastem N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose MyBioSource MBS652918 RPMI 1640 Medium without Glucose and L-Glutamine Sartorius 01-101-1A RPMI 1640 Medium without L-Glutamine Sartorius 01-104-1A Collagenase P Sigma 11213865001 Histopaque®-1077 Sigma 10771 Histopaque®-1119 Sigma 11191 HEPES buffer 1M Biological industries 03-025-1B Glutamine 200mM Biological industries 03-020-1A D (+)-Glucose Roth 50-99-7 Accutase Sigma A7089 Fetal bovine serum (FBS) Sigma F0926 Bovine serum albumin (BSA) Sigma A7906 BD Cytofix/CytopermTM Kit BD Biosciences 554714 Insulin Sigma I2643 S961 Novonordisk N/A Forskolin TCI F0855 Diazoxide Sigma D9035 Exendin-4 APExBIO A3408 Nifedipine Sigma N7634 Tolbutamide Sigma T0891 KCl BioLab 001638059100 GKA Pfizer HY-108328 Torin-1 EMD millipore 475991 (Continued on next page) Cell Reports 44, 115647, May 27, 2025 15 ..
Article Title: Ptprf is the conserved receptor for Asprosin's glucogenic effects in vertebrates.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151) (19H7) Rabbit mAb Cell Signaling Technology Cat# 3024T; RRID:AB_331253 Phospho-IGF-I Receptor beta (Tyr1131)/Insulin Receptor beta (Tyr1146) Antibody Cell Signaling Technology Cat# 3021T; RRID:AB_331578 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb Cell Signaling Technology Cat# 4060; RRID:AB_2315049 Rabbit Polyclonal Anti-β-actin Cell Signaling Technology Cat# 4967; RRID: AB_10695744 INSR Polyclonal antibody Proteintech Cat# 20433-1-AP; RRID:AB_10646469 AKT Polyclonal antibody Proteintech Cat# 10176-2-AP; RRID:AB_2224574 DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) Proteintech Cat# 20543-1-AP; RRID:AB_11232216 DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) Proteintech Cat# 66008-4-Ig; RRID:AB_2918475 HA tag Polyclonal antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 HA Tag Monoclonal antibody Proteintech Cat# 66006-2-Ig; RRID:AB_2881490 Anti-PTPRF Antibody BOSTER Cat# PB0814 HRP Conjugated Goat Anti-mouse IgG (H + L) Secondary Antibody Cell Signaling Technology Cat# 7076; RRID:AB_330924 HRP Conjugated Goat Anti-rabbit IgG (H + L) Secondary Antibody Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Alexa 488 Conjugated Goat Anti-rabbit IgG (H + L) CrossAdsorbed Secondary Antibody Thermo Fisher Cat# A-11008; RRID:AB_143165 Alexa 594 Conjugated Goat Anti-rabbit IgG (H + L) Secondary Antibody Thermo Fisher Cat# A-21207; RRID:AB_141637 Anti-Asprosin Antibody This paper N/A Bacterial and virus strains E.coli DH5α Competent Cells Takara Bio Cat# 9057 Chemicals, peptides, and recombinant proteins TRIzol Invitrogen Cat# 15596026 Recombinant GFP protein BIOSS Cat# bs-33009P LR ClonaseTM II Plus enzyme Invitrogen Cat# 12538200 Endonucleases NEB N/A Tissue-Tek® O.C.T. .. Compound Sakura Cat# 4583 Clarity Western ECL Substrate BioRad Cat# 1705061 BASIC DMEM, High Glucose Gibco Cat# 11965092 Fetal Bovine Serum Gibco Cat# A5256701 Penicillin-Streptomycin Gibco Cat# 15140148 Recombinant Human LAR Protein R&D systems Cat# 9377-PF Zebrafish Asprosin recombinant protein Genscript N/A Critical commercial assays PrimeScriptTM RT reagent Kit Takara Bio Cat# RR047A SYBR Green real-time PCR master mix kit Toyobo Cat# QPK-201 DIG RNA Labeling Kit Roche Cat# 11175025910 Guide-it Recombinant Cas9 Clontech Cat# 632641 Deposited data Proteomics data PRIDE (ProteomeXchange) PXD072402 (Continued on next page) Cell Reports 45, 116974, February 24, 2026 13
Inhibition:Article Title: AZ14289671 is a highly selective and blood-brain barrier penetrant irreversible TKI that targets EGFRExon20 insertions
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-EGF Receptor (Tyr1068) Antibody Cell Signaling Technology Cat#2234; RRID:AB_331701 EGF Receptor (D38B1) XP Rabbit mAb Cell Signaling Technology Cat#4267; RRID:AB_2246311 Phospho-p44/42 MAPK (Erk1/2) (Thr202/ Tyr204) Antibody Cell Signaling Technology Cat#9101; RRID:AB_331646 p44/42 MAPK (Erk1/2) Antibody Cell Signaling Technology Cat9102; RRID:AB_330744 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat#4060; RRID:AB_2315049 Akt Antibody Cell Signaling Technology Cat#9272; RRID:AB_329827 Phospho-S6 Ribosomal Protein (Ser235/ 236) (D57.2.2E) XP Rabbit mAb Cell Signaling Technology Cat#4858; RRID:AB_916156 S6 Ribosomal Protein (54D2) Mouse mAb Cell Signaling Technology Cat#2317; RRID:AB_2238583 Biological Samples LU3075 Crown Bioscience N/A LU0387 Crown Bioscience N/A Chemicals, peptides, and recombinant proteins AZ14289671 AstraZeneca N/A Mobocertinib AstraZeneca N/A Zipalertinib AstraZeneca N/A Sunvozertinib AstraZeneca N/A Critical commercial assays HTRF Human Phospho-EGFR (Tyr1068) Detection Kit Revvity Part #: 64EG1PEG HTRF Human and Mouse Phospho-ERK (Thr202/Tyr204) Detection Kit Revvity Part #: 64AERPET Deposited data Original code used to compare the growth rate inhibition or potency of the compounds tested This paper https://doi.org/10.5281/zenodo.15534119 Experimental models: Cell lines NCI-H2073 ATCC CRL-5918; RRID:CVCL_1521 LXF2478 Oncotest N/A .. HaCaT Professor Norbert E. Fusenig in Deutsches Krebsforschungszentrum (DKFZ) N/A NCI-H2073 EGFRD770-N771InsSVD AstraZeneca Generated as previously described by Floc’h et al.3 NCI-H2073 EGFRV769-D770InsASV AstraZeneca Generated as previously described by Floc’h et al.3 NCI-H2073 EGFRD770-N771InsNPG AstraZeneca Generated as previously described by Floc’h et al.3 Experimental models: Organisms/strains Mouse: SCID: C.B-17/IcrHanHsd-Prkdcscid Envigo UK N/A Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo UK N/A Mouse: Balb/c Nude: BALB/cNj-Foxn1nu/ Gpt GemPharmatech Co., Ltd N/A (Continued on next page) Cell Reports Medicine 6, 102305, September 16, 2025 e1 Article ll OPEN ACCESS
other:Article Title: Regulation of Type 1 Diabetes via Brown Adipocyte-Secreted Proteins and the Novel Glucagon Regulator Nidogen-2.
Article Snippet: Current treatments for type 1 diabetes (T1D) focus on insulin replacement.. We demonstrate the therapeutic potential of a secreted protein fraction from embryonic brown adipose tissue (BAT) that mediates insulin receptor-dependent recovery of euglycemia in a T1D model, nonobese diabetic (NOD) mice, by suppressing glucagon secretion.. This fraction promotes white adipocyte differentiation and browning, maintains healthy BAT, and enhances glucose uptake in adipose tissue, skeletal muscle, and liver.
Purification:Article Title: Beta cells intrinsically sense and limit their secretory activity via mTORC1-RhoA signaling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig anti-insulin Primary Agilent Cat# IR002, RRID:AB_2800361 Mouse anti-glucagon Santa Cruz Biotechnology Cat# sc-514592; RRID:AB_2904166 Rabbit anti-phospho-S6 ribosomal protein (Ser240/244) Cell Signaling Technology Cat# 5364; RRID:AB_10694233 Rat anti-puromycin Antibody, clone 17H1 Sigma-Aldrich Cat# MABE341, RRID:AB_3677409 Rabbit phospho-Myosin Light Chain 2 (Ser19) Antibody Cell Signaling Technology Cat# 3671, RRID:AB_330248 Rabbit phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb Cell Signaling Technology Cat# 2855; RRID:AB_560835 Mouse anti-F-actin antibody [NH3] Abcam Cat# ab205; RRID:AB_302794 Phalloidin-iFluor 594 Abcam Cat# ab176757; RRID: AB_3695680 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb Cell Signaling Technology Cat# 4060, RRID:AB_2315049 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb Cell Signaling Technology Cat# 2535, RRID:AB_331250 Purified Mouse Anti-E-Cadherin BD Biosciences Cat# 610181, RRID:AB_397580 Biological samples Cadaveric human islets Prodo labs N/A SC-islets Kadimastem N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose MyBioSource MBS652918 RPMI 1640 Medium without Glucose and L-Glutamine Sartorius 01-101-1A RPMI 1640 Medium without L-Glutamine Sartorius 01-104-1A Collagenase P Sigma 11213865001 Histopaque®-1077 Sigma 10771 Histopaque®-1119 Sigma 11191 HEPES buffer 1M Biological industries 03-025-1B Glutamine 200mM Biological industries 03-020-1A D (+)-Glucose Roth 50-99-7 Accutase Sigma A7089 Fetal bovine serum (FBS) Sigma F0926 Bovine serum albumin (BSA) Sigma A7906 BD Cytofix/CytopermTM Kit BD Biosciences 554714 Insulin Sigma I2643 S961 Novonordisk N/A Forskolin TCI F0855 Diazoxide Sigma D9035 Exendin-4 APExBIO A3408 Nifedipine Sigma N7634 Tolbutamide Sigma T0891 KCl BioLab 001638059100 GKA Pfizer HY-108328 Torin-1 EMD millipore 475991 (Continued on next page) Cell Reports 44, 115647, May 27, 2025 15 ..
Modification:Article Title: Beta cells intrinsically sense and limit their secretory activity via mTORC1-RhoA signaling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig anti-insulin Primary Agilent Cat# IR002, RRID:AB_2800361 Mouse anti-glucagon Santa Cruz Biotechnology Cat# sc-514592; RRID:AB_2904166 Rabbit anti-phospho-S6 ribosomal protein (Ser240/244) Cell Signaling Technology Cat# 5364; RRID:AB_10694233 Rat anti-puromycin Antibody, clone 17H1 Sigma-Aldrich Cat# MABE341, RRID:AB_3677409 Rabbit phospho-Myosin Light Chain 2 (Ser19) Antibody Cell Signaling Technology Cat# 3671, RRID:AB_330248 Rabbit phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb Cell Signaling Technology Cat# 2855; RRID:AB_560835 Mouse anti-F-actin antibody [NH3] Abcam Cat# ab205; RRID:AB_302794 Phalloidin-iFluor 594 Abcam Cat# ab176757; RRID: AB_3695680 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb Cell Signaling Technology Cat# 4060, RRID:AB_2315049 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb Cell Signaling Technology Cat# 2535, RRID:AB_331250 Purified Mouse Anti-E-Cadherin BD Biosciences Cat# 610181, RRID:AB_397580 Biological samples Cadaveric human islets Prodo labs N/A SC-islets Kadimastem N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose MyBioSource MBS652918 RPMI 1640 Medium without Glucose and L-Glutamine Sartorius 01-101-1A RPMI 1640 Medium without L-Glutamine Sartorius 01-104-1A Collagenase P Sigma 11213865001 Histopaque®-1077 Sigma 10771 Histopaque®-1119 Sigma 11191 HEPES buffer 1M Biological industries 03-025-1B Glutamine 200mM Biological industries 03-020-1A D (+)-Glucose Roth 50-99-7 Accutase Sigma A7089 Fetal bovine serum (FBS) Sigma F0926 Bovine serum albumin (BSA) Sigma A7906 BD Cytofix/CytopermTM Kit BD Biosciences 554714 Insulin Sigma I2643 S961 Novonordisk N/A Forskolin TCI F0855 Diazoxide Sigma D9035 Exendin-4 APExBIO A3408 Nifedipine Sigma N7634 Tolbutamide Sigma T0891 KCl BioLab 001638059100 GKA Pfizer HY-108328 Torin-1 EMD millipore 475991 (Continued on next page) Cell Reports 44, 115647, May 27, 2025 15 ..
Gentle:Article Title: PTEN regulates vagal-insulin signaling to optimize autonomic output determining peripheral inflammatory and metabolic homeostasis.
Article Snippet: Protein lysates (30 μg) were fractionated by 10% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis followed by transfer onto ethanol-hydrated polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) for 10 minutes. .. Membranes were blocked for 1 hour with 5% non-fat dry milk, and then treated with antibodies against either tyrosine hydroxylase (1:1000, AB#152; Sigma Aldrich), or p-AKT (1:1000, Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060; CST), or total AKT (1:1000, Akt Antibody #9272; CST) at 4°C with gentle shaking overnight. .. Membranes were washed with TBST for 15 minutes total (3 x 5 minutes) and subsequently treated with anti-rabbit (CST#7074; 1:5000; CST) IgG horseradishperoxidase (HRP)-conjugated secondary antibody for 1 hour with gentle shaking.
Article Title: PTEN regulates vagal-insulin signaling to optimize autonomic output determining peripheral inflammatory and metabolic homeostasis
Article Snippet: Tissue lysates were mechanically homogenized for 3 cycles at 15 s intervals in ice-cold RIPA buffer (Thermo Fisher) containing protease and phosphatase inhibitors (CST#5872S), and centrifuged for 10 minutes at 14,000 × g at 4 °C to remove the lipid layer. .. Protein lysates (30 μg) were fractionated by 10% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis followed by transfer onto ethanol-hydrated polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) for 10 min. Membranes were blocked for 1 h with 5% non-fat dry milk, and then treated with antibodies against either tyrosine hydroxylase (1:1000, AB#152; Sigma Aldrich), or p-AKT (1:1000, Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060; CST), or total AKT (1:1000, Akt Antibody #9272; CST) at 4 °C with gentle shaking overnight. .. Membranes were washed with TBST for 15 min total (3 × 5 min) and subsequently treated with anti-rabbit (CST#7074; 1:5000; CST) IgG horseradish-peroxidase (HRP)-conjugated secondary antibody for 1 h with gentle shaking.
Nucleic Acid Electrophoresis:Article Title: PTEN regulates vagal-insulin signaling to optimize autonomic output determining peripheral inflammatory and metabolic homeostasis
Article Snippet: Tissue lysates were mechanically homogenized for 3 cycles at 15 s intervals in ice-cold RIPA buffer (Thermo Fisher) containing protease and phosphatase inhibitors (CST#5872S), and centrifuged for 10 minutes at 14,000 × g at 4 °C to remove the lipid layer. .. Protein lysates (30 μg) were fractionated by 10% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis followed by transfer onto ethanol-hydrated polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) for 10 min. Membranes were blocked for 1 h with 5% non-fat dry milk, and then treated with antibodies against either tyrosine hydroxylase (1:1000, AB#152; Sigma Aldrich), or p-AKT (1:1000, Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060; CST), or total AKT (1:1000, Akt Antibody #9272; CST) at 4 °C with gentle shaking overnight. .. Membranes were washed with TBST for 15 min total (3 × 5 min) and subsequently treated with anti-rabbit (CST#7074; 1:5000; CST) IgG horseradish-peroxidase (HRP)-conjugated secondary antibody for 1 h with gentle shaking.
FLAG-tag:Article Title: Ptprf is the conserved receptor for Asprosin's glucogenic effects in vertebrates.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151) (19H7) Rabbit mAb Cell Signaling Technology Cat# 3024T; RRID:AB_331253 Phospho-IGF-I Receptor beta (Tyr1131)/Insulin Receptor beta (Tyr1146) Antibody Cell Signaling Technology Cat# 3021T; RRID:AB_331578 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb Cell Signaling Technology Cat# 4060; RRID:AB_2315049 Rabbit Polyclonal Anti-β-actin Cell Signaling Technology Cat# 4967; RRID: AB_10695744 INSR Polyclonal antibody Proteintech Cat# 20433-1-AP; RRID:AB_10646469 AKT Polyclonal antibody Proteintech Cat# 10176-2-AP; RRID:AB_2224574 DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) Proteintech Cat# 20543-1-AP; RRID:AB_11232216 DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) Proteintech Cat# 66008-4-Ig; RRID:AB_2918475 HA tag Polyclonal antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 HA Tag Monoclonal antibody Proteintech Cat# 66006-2-Ig; RRID:AB_2881490 Anti-PTPRF Antibody BOSTER Cat# PB0814 HRP Conjugated Goat Anti-mouse IgG (H + L) Secondary Antibody Cell Signaling Technology Cat# 7076; RRID:AB_330924 HRP Conjugated Goat Anti-rabbit IgG (H + L) Secondary Antibody Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Alexa 488 Conjugated Goat Anti-rabbit IgG (H + L) CrossAdsorbed Secondary Antibody Thermo Fisher Cat# A-11008; RRID:AB_143165 Alexa 594 Conjugated Goat Anti-rabbit IgG (H + L) Secondary Antibody Thermo Fisher Cat# A-21207; RRID:AB_141637 Anti-Asprosin Antibody This paper N/A Bacterial and virus strains E.coli DH5α Competent Cells Takara Bio Cat# 9057 Chemicals, peptides, and recombinant proteins TRIzol Invitrogen Cat# 15596026 Recombinant GFP protein BIOSS Cat# bs-33009P LR ClonaseTM II Plus enzyme Invitrogen Cat# 12538200 Endonucleases NEB N/A Tissue-Tek® O.C.T. .. Compound Sakura Cat# 4583 Clarity Western ECL Substrate BioRad Cat# 1705061 BASIC DMEM, High Glucose Gibco Cat# 11965092 Fetal Bovine Serum Gibco Cat# A5256701 Penicillin-Streptomycin Gibco Cat# 15140148 Recombinant Human LAR Protein R&D systems Cat# 9377-PF Zebrafish Asprosin recombinant protein Genscript N/A Critical commercial assays PrimeScriptTM RT reagent Kit Takara Bio Cat# RR047A SYBR Green real-time PCR master mix kit Toyobo Cat# QPK-201 DIG RNA Labeling Kit Roche Cat# 11175025910 Guide-it Recombinant Cas9 Clontech Cat# 632641 Deposited data Proteomics data PRIDE (ProteomeXchange) PXD072402 (Continued on next page) Cell Reports 45, 116974, February 24, 2026 13
Virus:Article Title: Ptprf is the conserved receptor for Asprosin's glucogenic effects in vertebrates.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151) (19H7) Rabbit mAb Cell Signaling Technology Cat# 3024T; RRID:AB_331253 Phospho-IGF-I Receptor beta (Tyr1131)/Insulin Receptor beta (Tyr1146) Antibody Cell Signaling Technology Cat# 3021T; RRID:AB_331578 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb Cell Signaling Technology Cat# 4060; RRID:AB_2315049 Rabbit Polyclonal Anti-β-actin Cell Signaling Technology Cat# 4967; RRID: AB_10695744 INSR Polyclonal antibody Proteintech Cat# 20433-1-AP; RRID:AB_10646469 AKT Polyclonal antibody Proteintech Cat# 10176-2-AP; RRID:AB_2224574 DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) Proteintech Cat# 20543-1-AP; RRID:AB_11232216 DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) Proteintech Cat# 66008-4-Ig; RRID:AB_2918475 HA tag Polyclonal antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 HA Tag Monoclonal antibody Proteintech Cat# 66006-2-Ig; RRID:AB_2881490 Anti-PTPRF Antibody BOSTER Cat# PB0814 HRP Conjugated Goat Anti-mouse IgG (H + L) Secondary Antibody Cell Signaling Technology Cat# 7076; RRID:AB_330924 HRP Conjugated Goat Anti-rabbit IgG (H + L) Secondary Antibody Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Alexa 488 Conjugated Goat Anti-rabbit IgG (H + L) CrossAdsorbed Secondary Antibody Thermo Fisher Cat# A-11008; RRID:AB_143165 Alexa 594 Conjugated Goat Anti-rabbit IgG (H + L) Secondary Antibody Thermo Fisher Cat# A-21207; RRID:AB_141637 Anti-Asprosin Antibody This paper N/A Bacterial and virus strains E.coli DH5α Competent Cells Takara Bio Cat# 9057 Chemicals, peptides, and recombinant proteins TRIzol Invitrogen Cat# 15596026 Recombinant GFP protein BIOSS Cat# bs-33009P LR ClonaseTM II Plus enzyme Invitrogen Cat# 12538200 Endonucleases NEB N/A Tissue-Tek® O.C.T. .. Compound Sakura Cat# 4583 Clarity Western ECL Substrate BioRad Cat# 1705061 BASIC DMEM, High Glucose Gibco Cat# 11965092 Fetal Bovine Serum Gibco Cat# A5256701 Penicillin-Streptomycin Gibco Cat# 15140148 Recombinant Human LAR Protein R&D systems Cat# 9377-PF Zebrafish Asprosin recombinant protein Genscript N/A Critical commercial assays PrimeScriptTM RT reagent Kit Takara Bio Cat# RR047A SYBR Green real-time PCR master mix kit Toyobo Cat# QPK-201 DIG RNA Labeling Kit Roche Cat# 11175025910 Guide-it Recombinant Cas9 Clontech Cat# 632641 Deposited data Proteomics data PRIDE (ProteomeXchange) PXD072402 (Continued on next page) Cell Reports 45, 116974, February 24, 2026 13
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